The guaranteed shelf life from date of receipt for bioscience malignant histiocytoma kits is listed on the product information sheet. Some kits have an expiration date printed on the kit malignant histiocytoma box label, this is the guaranteed shelf life date calculated from the malignant histiocytoma day that the product shipped from our facility. Kits often are functional for significantly longer than the guaranteed malignant histiocytoma shelf life. If you have an older kit in storage that you malignant histiocytoma wish to use, we recommend performing a small scale positive control experiment to malignant histiocytoma confirm that the kit still works for your application before malignant histiocytoma processing a large number of samples or precious samples.
The guaranteed shelf life from date of receipt for antibodies malignant histiocytoma and conjugates is listed on the product information sheet. Antibodies and other conjugates often are functional for significantly longer malignant histiocytoma than the guaranteed shelf life. If you have an older conjugate in storage that you malignant histiocytoma wish to use, we recommend performing a small scale positive control experiment to malignant histiocytoma confirm that the product still works for your application before malignant histiocytoma processing a large number of samples or precious samples.
Biotium guarantees the stability of chemicals, dyes, and gel stains for at least a year from the malignant histiocytoma date you receive the product. However, the majority of these products are highly stable for many malignant histiocytoma years, as long as they are stored as recommended. Storage conditions can be found on the product information sheet malignant histiocytoma or product safety and data sheet, material safety data sheet, and on the product label. Fluorescent compounds should be protected from light for long term malignant histiocytoma storage.
Ester dyes are stable in solid form as long as malignant histiocytoma they are protected from light and moisture. Esters are not stable in aqueous solution. Concentrated stock solutions should be prepared in anhydrous DMSO (see biotium catalog no. 90082). Stock solutions in anhydrous DMSO can be stored desiccated at malignant histiocytoma -20°C for one month or longer. Esters should be diluted in aqueous solution immediately before use. Succinimidyl esters (SE) should be dissolved in a solution that is free of malignant histiocytoma amine-containing compounds like tris, glycine, or protein, which will react with the SE functional group. AM esters and diacetate compounds should be dissolved in a malignant histiocytoma solution that is free of serum, because serum could contain esterases that would hydrolyze the compound.
Succinimidyl esters are generally susceptible to hydrolysis, which can result in lower labeling efficiency. Heavily sulfonated dyes, such as the alexa fluor® dyes, dylight® dyes and irdyes® are particularly hygroscopic, worsening the hydrolysis problem. For example, the percent of active alexa fluor® 488 succinimidyl ester (SE) could be well below 50% by the time of application (according to the manufacturer’s product datasheet). In a number of alexa fluor® SE reactive dyes, the SE group is derived from an aromatic carboxylic acid, while in all of biotium’s CF™ dyes the SE group is prepared from an aliphatic carboxylic malignant histiocytoma acid. This structural difference reduces the susceptibility of CF™ dye SE reactive groups to hydrolysis, resulting in relatively stable reactive dyes with consistently higher labeling malignant histiocytoma efficiency compared to other SE derivatives of other fluorescent dyes.
Amines, aminooxy (also known as oxylamine), hydrazide, azide, alkyne, BCN, and tyramide reactive dyes, as well as dye free acids, are generally stable in aqueous solution when stored at -20°C for 6-12 months or longer, as long as no compounds are present that may react malignant histiocytoma with the dye’s functional group. See the product information sheets for specific reactive dyes more malignant histiocytoma information.
Coelenterazines are stable in solid form when stored as recommended; they are not stable in aqueous solution. Concentrated coelenterazine stock solutions (typically 1-100 mg/ml) should be prepared in ethanol or methanol; do not use DMSO or DMF to dissolve coelenterazines, because these solvents will oxidize the compounds. Ethanol or methanol stocks of coelenterazine can be stored at malignant histiocytoma -20°C or below for six months or longer; alcohol stocks may evaporate during storage, so use tightly sealing screw cap vials and wrap the malignant histiocytoma vials with parafilm for long term storage. Propylene glycol also can be used as a solvent to malignant histiocytoma minimize evaporation. If the solvent evaporates, the coelenterazine will still be present in the vial, so note the volume in the vial prior to storage malignant histiocytoma so that you can adjust the solvent volume to correct malignant histiocytoma for evaporation if needed. Prepare working solutions in aqueous buffers immediately before use. Coelenterazines are stable for up to five hours in aqueous malignant histiocytoma solution.
D-luciferin is stable in solid form and as a concentrated malignant histiocytoma stock solution when stored as recommended; it is not stable at dilute working concentrations in aqueous malignant histiocytoma solution. Prepare concentrated D-luciferin stock solutions (typically 1-100 mg/ml) in water, and store in aliquots at -20°C or below for six months or longer. Prepare working solutions immediately before use.
In western blotting, this antibody detects an antigen of 125 kda in human malignant histiocytoma liver and 135 kda in tumors of histiocytic origin. Comparative study of this mab and a standard CD68 mab malignant histiocytoma showed that their antigens are different. Its antigen in all macrophage types studied is located on malignant histiocytoma the plasma membrane and within cytoplasmic structures including lysosomes. This mab shows a restricted reactivity to cells of the malignant histiocytoma monocyte/macrophage system. It specifically reacts with blood monocytes and stains resident macrophages malignant histiocytoma in a wide variety of human tissues. This mab does not stain antigen-presenting cells, e.G., langerhans cells. Reportedly, its reactivity is restricted to histiocytes and macrophages.
Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® dye brochure for more information. Note: conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher malignant histiocytoma non-specific background than other dye colors.
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